Structural Biology Core

Charge per mixture

Maximum amount: 400

Experiment time on the 500 MHz NMR spectrometer, set up and processed by the user.

There will be a minimum of $25 per login session irrespective of the spectrometer usage time. So, whichever is the greater amount ($25 or the usage time multiplied by the dollar per hour) will be appearing as the charge.

Experiment time on the 700 MHz NMR spectrometer

MonoQ ion exchange, MonoS ion exchange

per plate, imaging included

Optimization of an initial crystal hit condition using additive reagents in a 96-well plate that accepts up to three proteins per well.  Available additive screens include Hampton Research Additive Screen, Qiagen Opti Salt, and Jena Bioscience JBScreen Plus Additives.

Services include: 1) Obligatory training on Vitrobot or Leica for new user. 2) Optional training for grid handling and sample preparation. 3) Grid preparation by facility manager (twice weekly, pending availability). 4) Consultation during office hours for data processing and structure determination.

Includes various Quantifoil, UltrAuFoil, and C-flat grids, as well as grids with continuous support (graphene oxide and ultrathin carbon)

Market price - Please contact

per plate

Grid screen optimization of initial crystal hit conditions in a 24-well plate that accepts up to four proteins per well.

per plate, imaging included

Crystal screening using 96 conditions.  The microbatch technique mixes protein solution and a crystallization reagent solution in a small volume to be covered with oil.  It is recommended to add up to 30% glycerol with the protein solution for this method. A complete setup consists of 18 commercial screens incubated at 22°C.

per plate, imaging included

Crystal screening using 96 conditions in a plate that accepts up to three proteins per well.  A complete setup consists of 18 commercial screens incubated at 22°C and 18 plates incubated at 4°C.

per hour

Obligate charge for each grid loaded in UTHSA cryo-TEM Glacios. Cost includes C-clip rings, C-clips, and labor.

Charge for isotopic labeling used in protein expression (15N, 13C or 2H)

Experiment time on the Jasco CD spectrometer

Refeyn TwoMP mass photometry mass and dispersity analysis.

Plasmid preparation and verification

per hour

setup, processing, and analysis

Required for 500 usage 

construction of plasmid and/or large scale expression

Protein construct design optimized for application. Custom gene synthesis with codon optimization for the expression system(s) of choice will be outsourced and priced based on sequence length.

Protein refolding protocol applied prior to purification steps

per day

Cryo screening or data collection of single particle data. Facility manager will prepare microscope for data collection. User will be responsible for screening and data collection. *Includes 24 hours of instrument time, 2 hours of staff assistance, and materials

Charged per compound

Maximum: 2000

Charged per compound

Maximum: 2000

Structure determination of a similar structure in parallel with a primary structure.

Phasing by heavy atom methods, model building, refinement, structure validation and Protein Data Bank deposition for model coordinates.

Phasing by molecular replacement, model building, refinement, structure validation and Protein Data Bank deposition for model coordinates.

Cryo screening or data collection of single particle data. Facility manager will prepare microscope for data collection, perform grid screening, and set up data collection. *Includes 24 hours of instrument time, 8 hours of staff time, and materials

Cryo screening of up to 5 grids. Note this is scheduled for every other Monday. *Includes 4 hours of instrument time, 4 hours of staff time, and materials

Cryo screening of up to 5 grids. Note this is scheduled for every other Monday. *Includes 4 hours of instrument time (plus optional overnight data collection), 4 hours of staff time, and materials

Cryo screening of up to 5 grids. Note this is scheduled for every other Monday. *Includes 4 hours of instrument time (plus optional overnight data collection), 4 hours of staff time, and materials

per beamtime shift

Nickel or glutathione affinity purification followed by size exclusion chromatography.

Trained users only

Setup fee added to each X-ray data collection

per hour 

X-ray diffraction data acquisition. There will be a $50 setup fee per data collection.

Preparation of a xenon derivative crystal (up to 500 psi)

Services include: 1) Obligatory training on Vitrobot or Leica for new user. 2) Optional training for grid handling and sample preparation. 3) Grid preparation by facility manager (twice weekly, pending availability). 4) Consultation during office hours for data processing and structure determination.

Includes various Quantifoil, UltrAuFoil, and C-flat grids, as well as grids with continuous support (graphene oxide and ultrathin carbon)

Market price - Please contact

Obligate charge for each grid loaded in UTHSA cryo-TEM Glacios. Cost includes C-clip rings, C-clips, and labor.

Cryo screening or data collection of single particle data. Facility manager will prepare microscope for data collection. User will be responsible for screening and data collection. *Includes 24 hours of instrument time, 2 hours of staff assistance, and materials

Cryo screening or data collection of single particle data. Facility manager will prepare microscope for data collection, perform grid screening, and set up data collection. *Includes 24 hours of instrument time, 8 hours of staff time, and materials

Cryo screening of up to 5 grids. Note this is scheduled for every other Monday. *Includes 4 hours of instrument time, 4 hours of staff time, and materials

Cryo screening of up to 5 grids. Note this is scheduled for every other Monday. *Includes 4 hours of instrument time (plus optional overnight data collection), 4 hours of staff time, and materials

Cryo screening of up to 5 grids. Note this is scheduled for every other Monday. *Includes 4 hours of instrument time (plus optional overnight data collection), 4 hours of staff time, and materials

Trained users only

Charge per mixture

Maximum amount: 400

Charged per compound

Maximum: 2000

Charged per compound

Maximum: 2000

Experiment time on the 500 MHz NMR spectrometer, set up and processed by the user.

There will be a minimum of $25 per login session irrespective of the spectrometer usage time. So, whichever is the greater amount ($25 or the usage time multiplied by the dollar per hour) will be appearing as the charge.

Experiment time on the 700 MHz NMR spectrometer

Charge for isotopic labeling used in protein expression (15N, 13C or 2H)

Experiment time on the Jasco CD spectrometer

setup, processing, and analysis

Required for 500 usage 

construction of plasmid and/or large scale expression

MonoQ ion exchange, MonoS ion exchange

per plate, imaging included

Optimization of an initial crystal hit condition using additive reagents in a 96-well plate that accepts up to three proteins per well.  Available additive screens include Hampton Research Additive Screen, Qiagen Opti Salt, and Jena Bioscience JBScreen Plus Additives.

per plate

Grid screen optimization of initial crystal hit conditions in a 24-well plate that accepts up to four proteins per well.

per plate, imaging included

Crystal screening using 96 conditions.  The microbatch technique mixes protein solution and a crystallization reagent solution in a small volume to be covered with oil.  It is recommended to add up to 30% glycerol with the protein solution for this method. A complete setup consists of 18 commercial screens incubated at 22°C.

per plate, imaging included

Crystal screening using 96 conditions in a plate that accepts up to three proteins per well.  A complete setup consists of 18 commercial screens incubated at 22°C and 18 plates incubated at 4°C.

per hour

Refeyn TwoMP mass photometry mass and dispersity analysis.

Plasmid preparation and verification

per hour

Protein construct design optimized for application. Custom gene synthesis with codon optimization for the expression system(s) of choice will be outsourced and priced based on sequence length.

Protein refolding protocol applied prior to purification steps

per day

Structure determination of a similar structure in parallel with a primary structure.

Phasing by heavy atom methods, model building, refinement, structure validation and Protein Data Bank deposition for model coordinates.

Phasing by molecular replacement, model building, refinement, structure validation and Protein Data Bank deposition for model coordinates.

per beamtime shift

Nickel or glutathione affinity purification followed by size exclusion chromatography.

Setup fee added to each X-ray data collection

per hour 

X-ray diffraction data acquisition. There will be a $50 setup fee per data collection.

Preparation of a xenon derivative crystal (up to 500 psi)