VPR-IT@UTHSCSA.EDU | +1 (210) 450-6666 | Submit a Ticket
(hide this warning on this page)The Structural Biology Core Laboratory has state-of-the-art instrumentation and expert staff to enable detailed structural and biophysical studies of biological macromolecules in human health and disease. Existing strengths include X-ray crystallography and NMR spectroscopy in addition to resources and infrastructure to assist users with protein production and characterization. A new cryo-EM facility for single particle analysis and structure determination is being built and is projected to go online in Spring 2022.
Publications and presentations which result from data collected in our facility must acknowledge our grant support. Without this support, we would be forced to charge higher fees for instrument time and core services. Please include the following in your acknowledgements: “We acknowledge the Structural Biology Core at the University of Texas Health Science Center at San Antonio which is supported by the Cancer Therapy and Research Center through the NCI P30 Grant CA054174 and Texas State funds provided through the Office of the Vice President for Research of UT Health at San Antonio.”
Shaun K. Olsen, Ph.D., SBC Director
Phone: (210) 450-3091
Email: OlsenS@uthscsa.edu
Alex B. Taylor, Ph.D., Associate Director
Phone: (210) 567-3781
Email: TaylorAB@uthscsa.edu
Kristin E. Cano, Ph.D., Technical Director
Phone: (210) 567-8706
Email: CanoK@uthscsa.edu
Hours | Location |
Monday - Friday 9:00am-6:00pm |
UT Health SA, Long Campus |
Name | Role | Phone | Location | |
---|---|---|---|---|
Shaun Olsen, Ph.D. |
Associate Professor, Director
|
(210) 450-3091
|
OlsenS@uthscsa.edu
|
|
Alex Taylor, Ph.D. |
Assistant Professor/Research, Associate Director
|
(210) 567-3781
|
TaylorAB@uthscsa.edu
|
MED 4.420D
|
Kristin Cano, Ph.D. |
Assistant Professor/Research, Technical Director
|
(210) 567-8706
|
CanoK@uthscsa.edu
|
RAB 5.210, MED 4.420D.2
|
Service list |
► Fragment Based Screening (HSQC) (1) | |||
Name | Description | Price | |
---|---|---|---|
5 - compound mixtures |
Charge per mixture Maximum amount: 400
|
Inquire | |
► X-Ray Crystallography Services (12) | |||
Name | Description | Price | |
Additive Screen |
per plate, imaging included
Optimization of an initial crystal hit condition using additive reagents in a 96-well plate that accepts up to three proteins per well. Available additive screens include Hampton Research Additive Screen, Qiagen Opti Salt, and Jena Bioscience JBScreen Plus Additives. |
Inquire | |
Crystal Optimization, Vapor Diffusion |
per plate
Grid screen optimization of initial crystal hit conditions in a 24-well plate that accepts up to four proteins per well. |
Inquire | |
Crystal Screen, Microbatch |
per plate, imaging included
Crystal screening using 96 conditions. The microbatch technique mixes protein solution and a crystallization reagent solution in a small volume to be covered with oil. It is recommended to add up to 30% glycerol with the protein solution for this method. A complete setup consists of 18 commercial screens incubated at 22°C. |
Inquire | |
Crystal Screen, Vapor Diffusion |
per plate, imaging included
Crystal screening using 96 conditions in a plate that accepts up to three proteins per well. A complete setup consists of 18 commercial screens incubated at 22°C and 18 plates incubated at 4°C. |
Inquire | |
Plasmid Construct Design |
Protein construct design optimized for application. Custom gene synthesis with codon optimization for the expression system(s) of choice will be outsourced and priced based on sequence length. |
Inquire | |
Structure Determination - Additional Structure |
Structure determination of a similar structure in parallel with a primary structure. |
Inquire | |
Structure Determination - Heavy Atom Phasing |
Phasing by heavy atom methods, model building, refinement, structure validation and Protein Data Bank deposition for model coordinates. |
Inquire | |
Structure Determination - Molecular Replacement |
Phasing by molecular replacement, model building, refinement, structure validation and Protein Data Bank deposition for model coordinates. |
Inquire | |
Synchrotron Data Collection/Analysis |
per beamtime shift |
Inquire | |
X-Ray Data Collection Setup Fee |
Setup fee added to each X-ray data collection |
Inquire | |
X-ray Diffraction Data Collection |
per hour
X-ray diffraction data acquisition. There will be a $50 setup fee per data collection. |
Inquire | |
Xenon Derivative |
Preparation of a xenon derivative crystal (up to 500 psi) |
Inquire |