X-Ray Crystallography

Overview of Services

The mission of the X-ray Crystallography Core Laboratory is to provide state-of-the-art resources to researchers at the University of Texas Health Science Center at San Antonio, and to external users, enabling the detailed 3-D analyses of biological macromolecules that play important roles in human health.

Leadership

Alex Taylor Director
Office: Medical Building 4.420D
Phone: 210-567-3781
Fax: 210-567-6595
Email: taylorab@uthscsa.edu

Ramiro Ramirez-Solis Director, Institutional Cores
Office: STRF 3.300
Phone: 210-567-2059
Email: ramirezsolis@uthscsa.edu

Location and hours of operation

Hours	Location
Monday - Friday 8:30am-4:30pm	7703 Floyd Curl Drive Medical Building 436C San Antonio, TX 78229

Links and Resources

X-ray Cryststallography Core Laboratory Website

Contacts

Name	Role	Phone	Email	Location
Alex Taylor, Ph.D.	Assistant Professor/Research, Director	210-567-3781	taylorab@uthscsa.edu	MED 4.420D

Service list

Name	Description	Price
Additive Screen	per plate, imaging included Optimization of an initial crystal hit condition using additive reagents in a 96-well plate that accepts up to three proteins per well. Available additive screens include Hampton Research Additive Screen, Qiagen Opti Salt, and Jena Bioscience JBScreen Plus Additives.	External – Academic $37.00 each
Crystal Optimization, Vapor Diffusion	per plate Grid screen optimization of initial crystal hit conditions in a 24-well plate that accepts up to four proteins per well.	External – Academic $42.00 each
Crystal Screen, Microbatch	per plate, imaging included Crystal screening using 96 conditions. The microbatch technique mixes protein solution and a crystallization reagent solution in a small volume to be covered with oil. It is recommended to add up to 30% glycerol with the protein solution for this method. A complete setup consists of 18 commercial screens incubated at 22°C.	External – Academic $42.00 each
Crystal Screen, Vapor Diffusion	per plate, imaging included Crystal screening using 96 conditions in a plate that accepts up to three proteins per well. A complete setup consists of 18 commercial screens incubated at 22°C and 18 plates incubated at 4°C.	External – Academic $42.00 each
Plasmid Construct Design	Protein construct design optimized for application. Custom gene synthesis with codon optimization for the expression system(s) of choice will be outsourced and priced based on sequence length.	External – Academic $120.00 each
Structure Determination - Additional Structure	Structure determination of a similar structure in parallel with a primary structure.	External – Academic $600.00 each
Structure Determination - Heavy Atom Phasing	Phasing by heavy atom methods, model building, refinement, structure validation and Protein Data Bank deposition for model coordinates.	External – Academic $4,000.00 each
Structure Determination - Molecular Replacement	Phasing by molecular replacement, model building, refinement, structure validation and Protein Data Bank deposition for model coordinates.	External – Academic $3,500.00 each
Synchrotron Data Collection/Analysis (Remote)	per beamtime shift	External – Academic $400.00 each
X-Ray Data Collection Setup Fee	Setup fee added to each X-ray data collection	External – Academic $50.00 each
X-ray Diffraction Data Collection	per hour X-ray diffraction data acquisition. There will be a $50 setup fee per data collection.	External – Academic $28.00 each
Xenon Derivative	Preparation of a xenon derivative crystal (up to 500 psi)	External – Academic $100.00 each

Search available services: View: by category alphabetically

Name	Description	Price
▼ ► Crystal Screen (4)
Additive Screen	per plate, imaging included Optimization of an initial crystal hit condition using additive reagents in a 96-well plate that accepts up to three proteins per well. Available additive screens include Hampton Research Additive Screen, Qiagen Opti Salt, and Jena Bioscience JBScreen Plus Additives.	External – Academic $37.00 each
Crystal Optimization, Vapor Diffusion	per plate Grid screen optimization of initial crystal hit conditions in a 24-well plate that accepts up to four proteins per well.	External – Academic $42.00 each
Crystal Screen, Microbatch	per plate, imaging included Crystal screening using 96 conditions. The microbatch technique mixes protein solution and a crystallization reagent solution in a small volume to be covered with oil. It is recommended to add up to 30% glycerol with the protein solution for this method. A complete setup consists of 18 commercial screens incubated at 22°C.	External – Academic $42.00 each
Crystal Screen, Vapor Diffusion	per plate, imaging included Crystal screening using 96 conditions in a plate that accepts up to three proteins per well. A complete setup consists of 18 commercial screens incubated at 22°C and 18 plates incubated at 4°C.	External – Academic $42.00 each
▼ ► Data Collection (3)
Name	Description	Price
Synchrotron Data Collection/Analysis (Remote)	per beamtime shift	External – Academic $400.00 each
X-Ray Data Collection Setup Fee	Setup fee added to each X-ray data collection	External – Academic $50.00 each
X-ray Diffraction Data Collection	per hour X-ray diffraction data acquisition. There will be a $50 setup fee per data collection.	External – Academic $28.00 each
▼ ► Other (2)
Name	Description	Price
Plasmid Construct Design	Protein construct design optimized for application. Custom gene synthesis with codon optimization for the expression system(s) of choice will be outsourced and priced based on sequence length.	External – Academic $120.00 each
Xenon Derivative	Preparation of a xenon derivative crystal (up to 500 psi)	External – Academic $100.00 each
▼ ► Structure Determination (3)
Name	Description	Price
Structure Determination - Additional Structure	Structure determination of a similar structure in parallel with a primary structure.	External – Academic $600.00 each
Structure Determination - Heavy Atom Phasing	Phasing by heavy atom methods, model building, refinement, structure validation and Protein Data Bank deposition for model coordinates.	External – Academic $4,000.00 each
Structure Determination - Molecular Replacement	Phasing by molecular replacement, model building, refinement, structure validation and Protein Data Bank deposition for model coordinates.	External – Academic $3,500.00 each