UTHSA X-Ray Crystallography

Overview of Services

The mission of the X-ray Crystallography Core Laboratory is to provide state-of-the-art resources to researchers at the University of Texas Health Science Center at San Antonio, and to external users, enabling the detailed 3-D analyses of biological macromolecules that play important roles in human health. This is a full service core in that it not only offers access to sophisticated equipment and technologies but also offers advice and technical assistance in sample preparation. Services are also provided to investigators without funding to facilitate the development of pilot data for new potentially fundable projects, as core resources permit.

Leadership

Alex Taylor Director
Office: Medical Building 545C
Phone: 210-567-3781
Fax: 210-567-6595
Email: taylorab@uthscsa.edu

Ramiro Ramirez-Solis Director, Institutional Cores
Office: STRF 3.300
Phone: 210-567-2059
Email: ramirezsolis@uthscsa.edu

Location and hours of operation

Hours	Location
Monday - Friday 8:30am-4:30pm	7703 Floyd Curl Drive Medical Building 436C San Antonio, TX 78229

Links and Resources

X-ray Cryststallography Core Laboratory Website

Contacts

Name	Role	Phone	Email	Location
Alex Taylor, Ph.D.	Assistant Professor/Research, Director	210-567-3781	taylorab@uthscsa.edu	MED 5.545C

Service list

Name	Description	Price
Additive Screen	per plate, imaging included Optimization of an initial crystal hit condition using additive reagents in a 96-well plate that accepts up to three proteins per well. Available additive screens include Hampton Research Additive Screen, Qiagen Opti Salt, and Jena Bioscience JBScreen Plus Additives. Art Robbins Instruments Phoenix Formulatrix RockImager	External – Academic $37.00 each
Crystal Optimization, Vapor Diffusion	per plate Grid screen optimization of initial crystal hit conditions in a 24-well plate that accepts up to four proteins per well. Art Robbins Instruments Scorpion	External – Academic $42.00 each
Crystal Screen, Microbatch	per plate, imaging included Crystal screening using 96 conditions. The microbatch technique mixes protein solution and a crystallization reagent solution in a small volume to be covered with oil. It is recommended to add up to 30% glycerol with the protein solution for this method. A complete setup consists of 18 commercial screens incubated at 22°C. Art Robbins Instruments Phoenix Formulatrix RockImager	External – Academic $42.00 each
Crystal Screen, Vapor Diffusion	per plate, imaging included Crystal screening using 96 conditions in a plate that accepts up to three proteins per well. A complete setup consists of 18 commercial screens incubated at 22°C and 18 plates incubated at 4°C. Art Robbins Instruments Phoenix Formulatrix RockImager	External – Academic $42.00 each
Structure Determination - Additional Structure	Structure determination of a similar structure in parallel with a primary structure.	External – Academic $600.00 each
Structure Determination - Heavy Atom Phasing	Phasing by heavy atom methods, model building, refinement, structure validation and Protein Data Bank deposition for model coordinates.	External – Academic $4,000.00 each
Structure Determination - Molecular Replacement	Phasing by molecular replacement, model building, refinement, structure validation and Protein Data Bank deposition for model coordinates.	External – Academic $3,500.00 each
Synchrotron Data Collection/Analysis (Remote)	per beamtime shift	External – Academic $400.00 each
X-Ray Data Collection Setup Fee	Setup fee added to each X-Ray data collection	External – Academic $50.00 each
X-ray Diffraction Data Collection	per hour X-ray diffraction data acquisition. There will be a $50 setup fee per data collection. UTHSCSA X-ray Diffractometer	External – Academic $28.00 each
Xenon Derivative	Preparation of a xenon derivative crystal (up to 500 psi)	External – Academic $100.00 each

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