X-Ray Crystallography

per plate, imaging included

Optimization of an initial crystal hit condition using additive reagents in a 96-well plate that accepts up to three proteins per well.  Available additive screens include Hampton Research Additive Screen, Qiagen Opti Salt, and Jena Bioscience JBScreen Plus Additives.

per plate

Grid screen optimization of initial crystal hit conditions in a 24-well plate that accepts up to four proteins per well.

per plate, imaging included

Crystal screening using 96 conditions.  The microbatch technique mixes protein solution and a crystallization reagent solution in a small volume to be covered with oil.  It is recommended to add up to 30% glycerol with the protein solution for this method. A complete setup consists of 18 commercial screens incubated at 22°C.

per plate, imaging included

Crystal screening using 96 conditions in a plate that accepts up to three proteins per well.  A complete setup consists of 18 commercial screens incubated at 22°C and 18 plates incubated at 4°C.

Protein construct design optimized for application. Custom gene synthesis with codon optimization for the expression system(s) of choice will be outsourced and priced based on sequence length.

Structure determination of a similar structure in parallel with a primary structure.

Phasing by heavy atom methods, model building, refinement, structure validation and Protein Data Bank deposition for model coordinates.

Phasing by molecular replacement, model building, refinement, structure validation and Protein Data Bank deposition for model coordinates.

per beamtime shift

Setup fee added to each X-ray data collection

per hour 

X-ray diffraction data acquisition. There will be a $50 setup fee per data collection.

Preparation of a xenon derivative crystal (up to 500 psi)

per plate, imaging included

Optimization of an initial crystal hit condition using additive reagents in a 96-well plate that accepts up to three proteins per well.  Available additive screens include Hampton Research Additive Screen, Qiagen Opti Salt, and Jena Bioscience JBScreen Plus Additives.

per plate

Grid screen optimization of initial crystal hit conditions in a 24-well plate that accepts up to four proteins per well.

per plate, imaging included

Crystal screening using 96 conditions.  The microbatch technique mixes protein solution and a crystallization reagent solution in a small volume to be covered with oil.  It is recommended to add up to 30% glycerol with the protein solution for this method. A complete setup consists of 18 commercial screens incubated at 22°C.

per plate, imaging included

Crystal screening using 96 conditions in a plate that accepts up to three proteins per well.  A complete setup consists of 18 commercial screens incubated at 22°C and 18 plates incubated at 4°C.

per beamtime shift

Setup fee added to each X-ray data collection

per hour 

X-ray diffraction data acquisition. There will be a $50 setup fee per data collection.

Protein construct design optimized for application. Custom gene synthesis with codon optimization for the expression system(s) of choice will be outsourced and priced based on sequence length.

Preparation of a xenon derivative crystal (up to 500 psi)

Structure determination of a similar structure in parallel with a primary structure.

Phasing by heavy atom methods, model building, refinement, structure validation and Protein Data Bank deposition for model coordinates.

Phasing by molecular replacement, model building, refinement, structure validation and Protein Data Bank deposition for model coordinates.