UTHSA Macromolecular Interactions

Overview of Services

The Macromolecular Interactions (MMI) Institutional Research Core Laboratory provides access to instrumentation and expertise for the complete characterization of a macromolecular interaction in solution. This includes Surface Plasmon Resonance (SPR), Isothermal Titration Calorimetry (ITC), Differential Scanning Calorimetry (DSC), Dynamic Light Scattering (DLS), Static Light Scattering (SLS), Analytical Ultracentrifugation (AUC) and Protein Expression and Purification (PEPcore) services. 

 

Services and Equipment:

Surface Plasmon Resonance (SPR) is used to study label-free molecular interactions in real time. SPR provides information on the kinetics and thermodynamics of macromolecular interactions in solution (association rate constants, dissociation rate constants, equilibrium constants) from the millimolar to picomolar range.  It has applications in basic biomedical research and in drug discovery. We provide access to BIAcore T200 and BIAcore 3000 Surface Plasmon Resonance instruments, and the expertise to design, perform and analyze experiments.

Isothermal Titration Calorimetry (ITC) can be used to measure sub-millimolar to nanomolar equilibrium constants (102 to 109 M-1) of macromolecular interactions in solution, as well as reaction stoichiometries. We provide access to a Microcal VP-ITC instrument, and the expertise to design, perform and analyze experiments.

Differential Scanning Calorimetry (DSC) DSC is a powerful technique to characterize temperature-induced conformational changes in proteins or other macromolecules. It is useful for measuring the melting temperature of a protein, which could be used to determine whether mutagenesis of a protein results in changes in its thermal stability (i.e. fold).  We provide access to a Microcal VP-DSC instrument, and the expertise to design, perform and analyze experiments.

Dynamic and Static Light Scattering  (DLS/SLS).  Light scattering can be used to get information on particle size, shape and heterogeneity.  The DynaPro NanoStar is available for Dynamic Light Scattering (DLS) measurements.  It is capable of measuring samples ranging in size from 0.5 nm to 1000 nm at concentrations as low as 0.1 mg/ml. The NanoStar also has a dedicated Static Light Scattering (SLS) detector so not only can it measure hydrodynamic radii, but absolute molecular weights as well.

Analytical Ultracentrifugation (AUC).   AUC can be used to get information on particle, size, shape and density.  We provide access to a Beckman Optima XLA analytical ultracentrifuge instrument. 

Protein Expression and Purification Services (PEPcore).  We provide access to a fully equipped biochemistry wet lab for the expression and purification of proteins from E. Coli expression systems, and the expertise to design, perform and analyze experiments.

Grant and manuscript preparation services are also available.

 

Leadership

Lafer, Eileen M. Scientific Director
Office: MED 415B
Phone: (210) 567-3764
Email: lafer@uthscsa.edu

 

Liping Wang, Technical Director

Office: MED 438B
Phone: (210) 567-0048
Email: wangL1@uthscsa.edu

 

 

Location and hours of operation

Hours Location

Monday - Friday 8:00am-5:00pm 

7703 Floyd Curl Dr
San Antonio, TX 78229-3900

Links and Resources

  1. Center for Macromolecular Interactions (CMMI) Webpage 

Contacts

Name Role Phone Email Location
Eileen M. Lafer, Ph.D.
Scientific Director
 
(210) 567-3764
 
lafer@uthscsa.edu
 
MED 415B
 
Liping Wang
Technical Director
 
(210) 567-0048
 
wangl1@biochem.uthscsa.edu
 
MED 438B
 

Service list


Search available services: View: by category alphabetically
LS/DSC/ITC (1)
SPR (5)

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