Established in late 2017, UT Health San Antonio Rodent Genome Engineering Core delivered its first genetically edited mouse line on January 2018. From a handful amount of received and completed Genetically Engineered Mouse Model (GEMM) system projects, we successfully cloned over 20 GEMM lines in 2019, and our success, commitment, and recognition are in constant growth.
Our services are summarized below.
GEMM Services: (Our goal is to provide the service requester with at least 2 to 3 founders; germline transmission can be verified for an additional fee)
Transgenic Mouse Service (up to 3 sessions of micro-injection/~300 transferred 2 cell embryos)
Materials: BAC or other linearized DNA
Delivery Method: Pronuclear microinjection
Default Strain: B6SJLF2
Gene Targeting Service* (up to 3 sessions of micro-injection/~300 transferred 2 cell embryos)
Materials: CRISPR RNP Complex with/without donor DNA
Subcloning the gene of interest (GOI), shRNA or gRNA into a related pAAV or Lentivirus based-plasmid.
Large-scale preparation of the pAAV or Lentivirus plasmids using Qiagen Endo-free Mega Prep kits (optional).
Large-scale transfection of involved plasmids into 40 x 15cm plates of HEK293 cells.
Harvest the AAV or Lentivirus production cells and purify virus.
Determine the titer of the viral stock (in genome copy number per ml, or GC/ml) through quantitative real-time PCR.
A typical titer higher than 10x13 GC/ml, and volumn for 500 ul, 1 ml, and 5 ml with different price upon quote.
Inquire
Customized Stable Cell Lines
Service include
Construct a viral vector containing your gene and desired selection marker
Generate virus
Transduce the cell line your desired
Select the stably transduced
Validate integration of the gene via genomic PCR
Validate the highest-expressing clone by Western Blot (if applicable)
Deliver two cryogenic vials of stable cells along with a final report
Inquire
Dry Shipper Loan
Requester is financially responsible for any damages caused to the dry shipper.
Inquire
Mouse Colony Maintenance
The Mouse Colony Maintenance Technician is responsible for the day-to-day management and care of laboratory mouse colonies. This role requires meticulous attention to detail to ensure the health and well-being of the animals, as well as the integrity of experimental data. Tasks include cage cleaning, animal identification, breeding management, and general husbandry duties.
Internal
$35.00
each
Sperm Cryo-Preservation
Up to 3 males per mouse line. QC includes sperm count motility score and post freeze-thaw IVF competency test.
Males should be between the ages of 4mo- 9mo, otherwise contact us.
**Please fill out "Sperm Cyropreservation Log" before submitting request**
Subcloning the gene of interest (GOI), shRNA or gRNA into a related pAAV or Lentivirus based-plasmid.
Large-scale preparation of the pAAV or Lentivirus plasmids using Qiagen Endo-free Mega Prep kits (optional).
Large-scale transfection of involved plasmids into 40 x 15cm plates of HEK293 cells.
Harvest the AAV or Lentivirus production cells and purify virus.
Determine the titer of the viral stock (in genome copy number per ml, or GC/ml) through quantitative real-time PCR.
A typical titer higher than 10x13 GC/ml, and volumn for 500 ul, 1 ml, and 5 ml with different price upon quote.
Inquire
Customized Stable Cell Lines
Service include
Construct a viral vector containing your gene and desired selection marker
Generate virus
Transduce the cell line your desired
Select the stably transduced
Validate integration of the gene via genomic PCR
Validate the highest-expressing clone by Western Blot (if applicable)
Deliver two cryogenic vials of stable cells along with a final report
Inquire
Dry Shipper Loan
Requester is financially responsible for any damages caused to the dry shipper.
Inquire
Mouse Colony Maintenance
The Mouse Colony Maintenance Technician is responsible for the day-to-day management and care of laboratory mouse colonies. This role requires meticulous attention to detail to ensure the health and well-being of the animals, as well as the integrity of experimental data. Tasks include cage cleaning, animal identification, breeding management, and general husbandry duties.
Internal
$35.00
each
Sperm Cryo-Preservation
Up to 3 males per mouse line. QC includes sperm count motility score and post freeze-thaw IVF competency test.
Males should be between the ages of 4mo- 9mo, otherwise contact us.
**Please fill out "Sperm Cyropreservation Log" before submitting request**
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