Rodent Genome Engineering Core

Overview of Services

Established in late 2017, UT Health San Antonio Rodent Genome Engineering Core delivered its first genetically edited mouse line on January 2018. From a handful amount of received and completed Genetically Engineered Mouse Model (GEMM) system projects, we successfully cloned over 20 GEMM lines in 2019, and our success, commitment, and recognition are in constant growth.

Our services are summarized below.

GEMM Services:
(Our goal is to provide the service requester with at least 2 to 3 founders; germline transmission can be verified for an additional fee)

Transgenic Mouse Service (up to 3 sessions of micro-injection/~300 transferred 2 cell embryos)

Materials: BAC or other linearized DNA
Delivery Method: Pronuclear microinjection
Default Strain: B6SJLF2

Gene Targeting Service* (up to 3 sessions of micro-injection/~300 transferred 2 cell embryos)

Materials: CRISPR RNP Complex with/without donor DNA
Delivery Method: Pronuclear microinjection/2 cell microinjection/electroporation
Default Strain: B6SJLF2 or B6D2F2

*includes project planning, crRNA toxicity test, In/Del check, and founder genotyping

Other Services:

In-Vitro Fertilization
Sperm Cryo-preservation
Rederivation
Genotyping
Molecular Cloning

As we move forward, we will be providing Rat Genome Engineering service as well in near future.
You can find more information on our website at:
https://lsom.uthscsa.edu/physiology/transgenic-facility/

Top pictures from left to right: Electroporation and Microinjection setup

Bottom pictures from left to right: Pronuclear and 2-cell microinjection

Leadership

Dr. Qian Shi | Scientific Director
shiq@uthscsa.edu
(210) 567-4381

Location and hours of operation

Hours

Location

Monday - Friday 8AM - 5PM

Med. 330C

7703 Floyd Curl Drive
San Antonio, TX 78229

Links and Resources

Contacts

Name	Role	Phone	Email	Location
Qian Shi, Ph.D.	Scientific Director	(210) 567-4381	shiq@uthscsa.edu	Med. Bldg. Rm 334.5D

Service list

Name	Description	Price
Custom Lentivirus/AAVs Production Service	The services include Subcloning the gene of interest (GOI), shRNA or gRNA into a related pAAV or Lentivirus based-plasmid. Large-scale preparation of the pAAV or Lentivirus plasmids using Qiagen Endo-free Mega Prep kits (optional). Large-scale transfection of involved plasmids into 40 x 15cm plates of HEK293 cells. Harvest the AAV or Lentivirus production cells and purify virus. Determine the titer of the viral stock (in genome copy number per ml, or GC/ml) through quantitative real-time PCR. A typical titer higher than 10x¹³ GC/ml, and volumn for 500 ul, 1 ml, and 5 ml with different price upon quote.	Inquire
Customized Stable Cell Lines	Service include Construct a viral vector containing your gene and desired selection marker Generate virus Transduce the cell line your desired Select the stably transduced Validate integration of the gene via genomic PCR Validate the highest-expressing clone by Western Blot (if applicable) Deliver two cryogenic vials of stable cells along with a final report	Inquire
Dry Shipper Loan	Requester is financially responsible for any damages caused to the dry shipper.	Inquire
Sperm Cryo-Preservation	Up to 3 males per mouse line. QC includes sperm count motility score and post freeze-thaw IVF competency test. Males should be between the ages of 4mo- 9mo, otherwise contact us. Please fill out "Sperm Cyropreservation Log" before submitting request	Inquire

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