Rodent Genome Engineering Core

The services include

1. Subcloning the gene of interest (GOI), shRNA or gRNA into a related pAAV or Lentivirus based-plasmid.
2. Large-scale preparation of the pAAV or Lentivirus plasmids using Qiagen Endo-free Mega Prep kits (optional).
3. Large-scale transfection of involved plasmids into 40 x 15cm plates of HEK293 cells.
4. Harvest the AAV or Lentivirus production cells and purify virus.
5. Determine the titer of the viral stock (in genome copy number per ml, or GC/ml) through quantitative real-time PCR.
6. A typical titer higher than 10x¹³ GC/ml, and volumn for 500 ul, 1 ml, and 5 ml with different price upon quote.

Service include

1. Construct a viral vector containing your gene and desired selection marker
2. Generate virus
3. Transduce the cell line your desired
4. Select the stably transduced
5. Validate integration of the gene via genomic PCR
6. Validate the highest-expressing clone by Western Blot (if applicable)
7. Deliver two cryogenic vials of stable cells along with a final report 

The Mouse Colony Maintenance Technician is responsible for the day-to-day management and care of laboratory mouse colonies. This role requires meticulous attention to detail to ensure the health and well-being of the animals, as well as the integrity of experimental data. Tasks include cage cleaning, animal identification, breeding management, and general husbandry duties.

Up to 3 males per mouse line. QC includes sperm count motility score and post freeze-thaw IVF competency test.

Males should be between the ages of 4mo- 9mo, otherwise contact us.

**Please fill out "Sperm Cyropreservation Log" before submitting request**

Our transgenic core facility offers a reliable Cryopreserved Sperm Shipping Service, ensuring the safe transport of frozen sperm samples for research and breeding purposes. Cryopreserved sperm will be securely placed in a dry shipper tank pre-charged with liquid nitrogen, maintaining ultra-low temperatures during transit. We facilitate shipping through FedEx or UPS, providing efficient and monitored transport to ensure sample integrity. Each shipment will include a return shipping slip, allowing for the seamless return of the dry shipper tank after use. Our service ensures the proper handling and delivery of cryopreserved sperm with strict adherence to industry standards.

International shipping will be evaluated based on destination.

The services include

1. Subcloning the gene of interest (GOI), shRNA or gRNA into a related pAAV or Lentivirus based-plasmid.
2. Large-scale preparation of the pAAV or Lentivirus plasmids using Qiagen Endo-free Mega Prep kits (optional).
3. Large-scale transfection of involved plasmids into 40 x 15cm plates of HEK293 cells.
4. Harvest the AAV or Lentivirus production cells and purify virus.
5. Determine the titer of the viral stock (in genome copy number per ml, or GC/ml) through quantitative real-time PCR.
6. A typical titer higher than 10x¹³ GC/ml, and volumn for 500 ul, 1 ml, and 5 ml with different price upon quote.

Service include

1. Construct a viral vector containing your gene and desired selection marker
2. Generate virus
3. Transduce the cell line your desired
4. Select the stably transduced
5. Validate integration of the gene via genomic PCR
6. Validate the highest-expressing clone by Western Blot (if applicable)
7. Deliver two cryogenic vials of stable cells along with a final report 

The Mouse Colony Maintenance Technician is responsible for the day-to-day management and care of laboratory mouse colonies. This role requires meticulous attention to detail to ensure the health and well-being of the animals, as well as the integrity of experimental data. Tasks include cage cleaning, animal identification, breeding management, and general husbandry duties.

Our transgenic core facility offers a reliable Cryopreserved Sperm Shipping Service, ensuring the safe transport of frozen sperm samples for research and breeding purposes. Cryopreserved sperm will be securely placed in a dry shipper tank pre-charged with liquid nitrogen, maintaining ultra-low temperatures during transit. We facilitate shipping through FedEx or UPS, providing efficient and monitored transport to ensure sample integrity. Each shipment will include a return shipping slip, allowing for the seamless return of the dry shipper tank after use. Our service ensures the proper handling and delivery of cryopreserved sperm with strict adherence to industry standards.

International shipping will be evaluated based on destination.

Up to 3 males per mouse line. QC includes sperm count motility score and post freeze-thaw IVF competency test.

Males should be between the ages of 4mo- 9mo, otherwise contact us.

**Please fill out "Sperm Cyropreservation Log" before submitting request**