Overview of Services
Established in late 2017, UT Health San Antonio Rodent Genome Engineering Core delivered its first genetically edited mouse line on January 2018. From a handful amount of received and completed Genetically Engineered Mouse Model (GEMM) system projects, we successfully cloned over 20 GEMM lines in 2019, and our success, commitment, and recognition are in constant growth.
Our services are summarized below.
GEMM Services: (Our goal is to provide the service requester with at least 2 to 3 founders; germline transmission can be verified for an additional fee)
Transgenic Mouse Service (up to 3 sessions of micro-injection/~300 transferred 2 cell embryos)
Materials: BAC or other linearized DNA
Delivery Method: Pronuclear microinjection
Default Strain: B6SJLF2
Gene Targeting Service* (up to 3 sessions of micro-injection/~300 transferred 2 cell embryos)
Materials: CRISPR RNP Complex with/without donor DNA
Delivery Method: Pronuclear microinjection/2 cell microinjection/electroporation
Default Strain: B6SJLF2 or B6D2F2
*includes project planning, crRNA toxicity test, In/Del check, and founder genotyping
Other Services:
In-Vitro Fertilization
Sperm Cryo-preservation
Rederivation
Genotyping
Molecular Cloning
As we move forward, we will be providing Rat Genome Engineering service as well in near future. You can find more information on our website at:https://lsom.uthscsa.edu/physiology/transgenic-facility/
Top pictures from left to right: Electroporation and Microinjection setup
Bottom pictures from left to right: Pronuclear and 2-cell microinjection
Leadership
Dr. Qian Shi | Scientific Director shiq@uthscsa.edu (210) 567-4381
Location and hours of operation
Hours
Location
Monday - Friday 8AM - 5PM
Med. 330C
7703 Floyd Curl Drive San Antonio, TX 78229
Links and Resources
https://genome.ucsc.edu/
http://useast.ensembl.org/index.html
https://www.addgene.org/
http://www.informatics.jax.org/
https://www.mousephenotype.org/
http://crispor.tefor.net/
Search available services:
View: by category
alphabetically
Name
Description
Price
Custom Lentivirus/AAVs Production Service
The services include
Subcloning the gene of interest (GOI), shRNA or gRNA into a related pAAV or Lentivirus based-plasmid.
Large-scale preparation of the pAAV or Lentivirus plasmids using Qiagen Endo-free Mega Prep kits (optional).
Large-scale transfection of involved plasmids into 40 x 15cm plates of HEK293 cells.
Harvest the AAV or Lentivirus production cells and purify virus.
Determine the titer of the viral stock (in genome copy number per ml, or GC/ml) through quantitative real-time PCR.
A typical titer higher than 10x13 GC/ml, and volumn for 500 ul, 1 ml, and 5 ml with different price upon quote.
Inquire
Customized Stable Cell Lines
Service include
Construct a viral vector containing your gene and desired selection marker
Generate virus
Transduce the cell line your desired
Select the stably transduced
Validate integration of the gene via genomic PCR
Validate the highest-expressing clone by Western Blot (if applicable)
Deliver two cryogenic vials of stable cells along with a final report
Inquire
Dry Shipper Loan
Requester is financially responsible for any damages caused to the dry shipper.
Inquire
Sperm Cryo-Preservation
Up to 3 males per mouse line. QC includes sperm count motility score and post freeze-thaw IVF competency test.
Males should be between the ages of 4mo- 9mo, otherwise contact us.
**Please fill out "Sperm Cyropreservation Log" before submitting request**
Inquire
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Other Services (4)
Name
Description
Price
Custom Lentivirus/AAVs Production Service
The services include
Subcloning the gene of interest (GOI), shRNA or gRNA into a related pAAV or Lentivirus based-plasmid.
Large-scale preparation of the pAAV or Lentivirus plasmids using Qiagen Endo-free Mega Prep kits (optional).
Large-scale transfection of involved plasmids into 40 x 15cm plates of HEK293 cells.
Harvest the AAV or Lentivirus production cells and purify virus.
Determine the titer of the viral stock (in genome copy number per ml, or GC/ml) through quantitative real-time PCR.
A typical titer higher than 10x13 GC/ml, and volumn for 500 ul, 1 ml, and 5 ml with different price upon quote.
Inquire
Customized Stable Cell Lines
Service include
Construct a viral vector containing your gene and desired selection marker
Generate virus
Transduce the cell line your desired
Select the stably transduced
Validate integration of the gene via genomic PCR
Validate the highest-expressing clone by Western Blot (if applicable)
Deliver two cryogenic vials of stable cells along with a final report
Inquire
Dry Shipper Loan
Requester is financially responsible for any damages caused to the dry shipper.
Inquire
Sperm Cryo-Preservation
Up to 3 males per mouse line. QC includes sperm count motility score and post freeze-thaw IVF competency test.
Males should be between the ages of 4mo- 9mo, otherwise contact us.
**Please fill out "Sperm Cyropreservation Log" before submitting request**
Inquire