Bioanalytics and Single-Cell Core

Click here for list of Services and Fees

Overview of Service

Recent advances in genomic, epigenomic, proteomic and biophysical analyses at the single-cell level have greatly improved our understanding of intra-tissue heterogeneity, characterization of exfoliated and circulating single cells, and therapeutic response.

At the Bioanalytics and Single-Cell (BASIC) Core, we provide cutting-edge multiscale single-cell omics pipelines and multiplex technologies for advancing the discovery of biomarkers and therapeutic targets of various disease types.

WE OFFER

Atomic Force Microscope - NanoWizard 5 for biophysical assessment of cells and ECM.
Helios mass cytometer(CyTOF) - for detection with up to 50 markers for single cells.
Lunaphore COMET - for iterative immunofluorescence staining with up to 40 antibodies per tissue section.

The BASiC core was established by the Cancer Prevention Research Institute of Texas: RP150600, and is currently supported by the Office of Research and Innovation at UTSA.

Main Core Leadership

Nameer Kirma Ph.D., Director

Phone: (210) 562-4068

Email: kirma@uthscsa.edu

Chiou-Miin Wang Ph.D., Technical Director
Office: STRF 257
Email: wangc10@uthscsa.edu

Specialized Core Leadership

Pawel A. Osmulski Ph.D., Atomic Force Microscope Manager

Phone: (210) 567-7259

Email: osmulski@uthscsa.edu

Location and hours of operation

Hours

Location

Monday–Friday

8AM-5PM

South Texas Research Facility
Lab #257 | Mail Code 8255
8403 Floyd Curl Drive
San Antonio, Texas 78229

Links and Resources

BASiC Website

Contacts

Name	Role	Phone	Email	Location
Nameer Kirma, Ph.D.	Director	(210) 562-4068	kirma@uthscsa.edu	STRF 257
Chiou-Miin Wang, Ph.D.	Technical Director	(210) 562-4068	wangc10@uthscsa.edu	STRF 257

Services & Rates

Name	Description	Price
AFM chemical imaging with modified probes		Inquire
AFM imaging: force volume, tapping, or single point measurements		Inquire
AFM Imaging: Topography		Inquire
AFM Imaging: Topography + Mechanical Properties		Inquire
AFM Mechanical Imaging (QNM)		Inquire
AFM specialized post acquisition analysis (Morphometry, Nanomechanical Properties)		Inquire
BioMark 48x48 chip run	Including one BioMark 48x48 chip, reagents and labor. Do not include primers.	Inquire
Biomark 96x96 chip run	Including one BioMark 96x96 chip, reagents and labor. Do not include primers.	Internal $1,800.00 each External – Academic $2,700.00 each External – Commercial $3,600.00 each UT System/In Network $1,800.00 each
Biomark Fluidigm - Taqman Gene Expression	Biomark Fluidigm - Taqman Gene Expression (inquire about individual probe cost) 96x96 IFC	Inquire
Biomark Fluidigm-Taqman Gene Expression	Biomark Fluidigm-Taqman Gene Expression (inquire about individual probe cost) 48x48 IFC	Inquire
Biomark machine use		Internal $200.00 each External – Academic $300.00 each External – Commercial $400.00 each UT System/In Network $400.00 each
C1 HT800 single-cell mRNA Seq (includes chip and reagent costs and labor)	C1 (Fluidigm) HT800 single-cell mRNA Seq - through pooled indexing of single-cell capture (Does not include library preparation) Note: Sequencing will be perfromed by the UTHSA Next Generation Sequencing Facility (additional costs apply)	Inquire
C1 Preamp Single-cell Processing for single-cell multiplexing PCR (includes chip, reagents and labor)	C1 cell capture and cDNA preamplification for subsequent single-cell PCR (Up to 96 cells per chip). Does not include Fluidigm Biomark HD processing and PCR (see the Biomark services for microfluidic based single-cell PCR).	Inquire
C1 Single-Cell SMART-seq Processing 96-cells for RNA-seq (includes C1 chip, C1 single-cell processing , SMART-seq prep reagents and labor)	-includes C1 chip, C1 single-cell processing (up to 96-cells), SMART-seq prep reagents and labor	Inquire
C1 Single-Cell SMART-seq Processing 96-cells for RNA-seq (includes C1 chip, C1 single-cell processing , SMART-seq prep reagents and labor, Barcoding Library Prep)	Includes C1 chip, C1 single-cell processing (up to 96-cells), SMART-seq prep reagents and labor Includes Barcoding and Library prep	Inquire
CyTOF mixed sample staining reagent use		Inquire
CyTOF training for sample staining or antibody conjugation per day	BASiC Staff will provide a one time training of staining of a single sample with corresponding conjugated antibodies	Inquire
CyTOF Antibody Conjugation per X-8 polymer reaction	Pricing for Antibody conjugation of a single antibody to a single metal tag Preformed by BASiC Staff Does not include antibody Price	Inquire
CyTOF Bioinformatics per sample	BASiC will provide basic bioInformatic analysis Inquire about details	Inquire
CyTOF Core Antibody Use per Antibody per Sample	Price per antibody per sample using BASiC Core conjugation antibodies Inquire for list of antibodies and tags	Internal $6.00 each External – Academic $9.00 each External – Commercial $12.00 each UT System/In Network $6.00 each
CyTOF Helios Run by staff per day	Run time for 6 Samples on the Helios CyTOF	Inquire
CyTOF Helios use by users per day	BASIC Staff will assist customers to run samples on Helios machine, and this charge is for each day.	Inquire
CyTOF Sample Staining by Staff per day	Staining of up to 6 samples	Internal $300.00 each External – Academic $450.00 each External – Commercial $600.00 each UT System/In Network $300.00 each
FlexMap 3D Reading 1- 96 well plate with assay prepration by BASiC	Reading 1 96 well plate/Assay + Preparation by BASiC Personnel (must purchase kit through BASIC) Price per 96-Well plate	Inquire
Spatial COMET common reagent use	Cyclic immunofluorescence for FFPE or frozen tissue sections.	Internal $100.00 each External – Academic $150.00 each External – Commercial $200.00 each UT System/In Network $100.00 each
Statistical analysis and Publication ready AFM images		Inquire

Search available services: View: by category alphabetically

Name	Description	Price
▼ ► Atomic Force Micrscopy (AFM) (7)
AFM chemical imaging with modified probes		Inquire
AFM imaging: force volume, tapping, or single point measurements		Inquire
AFM Imaging: Topography		Inquire
AFM Imaging: Topography + Mechanical Properties		Inquire
AFM Mechanical Imaging (QNM)		Inquire
AFM specialized post acquisition analysis (Morphometry, Nanomechanical Properties)		Inquire
Statistical analysis and Publication ready AFM images		Inquire
▼ ► Helios CyTOF (8)
Name	Description	Price
CyTOF mixed sample staining reagent use		Inquire
CyTOF training for sample staining or antibody conjugation per day	BASiC Staff will provide a one time training of staining of a single sample with corresponding conjugated antibodies	Inquire
CyTOF Antibody Conjugation per X-8 polymer reaction	Pricing for Antibody conjugation of a single antibody to a single metal tag Preformed by BASiC Staff Does not include antibody Price	Inquire
CyTOF Bioinformatics per sample	BASiC will provide basic bioInformatic analysis Inquire about details	Inquire
CyTOF Core Antibody Use per Antibody per Sample	Price per antibody per sample using BASiC Core conjugation antibodies Inquire for list of antibodies and tags	Internal $6.00 each External – Academic $9.00 each External – Commercial $12.00 each UT System/In Network $6.00 each
CyTOF Helios Run by staff per day	Run time for 6 Samples on the Helios CyTOF	Inquire
CyTOF Helios use by users per day	BASIC Staff will assist customers to run samples on Helios machine, and this charge is for each day.	Inquire
CyTOF Sample Staining by Staff per day	Staining of up to 6 samples	Internal $300.00 each External – Academic $450.00 each External – Commercial $600.00 each UT System/In Network $300.00 each
▼ ► Luminex FlexMap 3D (1)
Name	Description	Price
FlexMap 3D Reading 1- 96 well plate with assay prepration by BASiC	Reading 1 96 well plate/Assay + Preparation by BASiC Personnel (must purchase kit through BASIC) Price per 96-Well plate	Inquire
▼ ► Fluidigm Biomark HD (5)
Name	Description	Price
BioMark 48x48 chip run	Including one BioMark 48x48 chip, reagents and labor. Do not include primers.	Inquire
Biomark 96x96 chip run	Including one BioMark 96x96 chip, reagents and labor. Do not include primers.	Internal $1,800.00 each External – Academic $2,700.00 each External – Commercial $3,600.00 each UT System/In Network $1,800.00 each
Biomark Fluidigm - Taqman Gene Expression	Biomark Fluidigm - Taqman Gene Expression (inquire about individual probe cost) 96x96 IFC	Inquire
Biomark Fluidigm-Taqman Gene Expression	Biomark Fluidigm-Taqman Gene Expression (inquire about individual probe cost) 48x48 IFC	Inquire
Biomark machine use		Internal $200.00 each External – Academic $300.00 each External – Commercial $400.00 each UT System/In Network $400.00 each
▼ ► C1 single-cell processing (4)
Name	Description	Price
C1 HT800 single-cell mRNA Seq (includes chip and reagent costs and labor)	C1 (Fluidigm) HT800 single-cell mRNA Seq - through pooled indexing of single-cell capture (Does not include library preparation) Note: Sequencing will be perfromed by the UTHSA Next Generation Sequencing Facility (additional costs apply)	Inquire
C1 Preamp Single-cell Processing for single-cell multiplexing PCR (includes chip, reagents and labor)	C1 cell capture and cDNA preamplification for subsequent single-cell PCR (Up to 96 cells per chip). Does not include Fluidigm Biomark HD processing and PCR (see the Biomark services for microfluidic based single-cell PCR).	Inquire
C1 Single-Cell SMART-seq Processing 96-cells for RNA-seq (includes C1 chip, C1 single-cell processing , SMART-seq prep reagents and labor)	-includes C1 chip, C1 single-cell processing (up to 96-cells), SMART-seq prep reagents and labor	Inquire
C1 Single-Cell SMART-seq Processing 96-cells for RNA-seq (includes C1 chip, C1 single-cell processing , SMART-seq prep reagents and labor, Barcoding Library Prep)	Includes C1 chip, C1 single-cell processing (up to 96-cells), SMART-seq prep reagents and labor Includes Barcoding and Library prep	Inquire
▼ ► Spatial Proteomics COMET System (1)
Name	Description	Price
Spatial COMET common reagent use	Cyclic immunofluorescence for FFPE or frozen tissue sections.	Internal $100.00 each External – Academic $150.00 each External – Commercial $200.00 each UT System/In Network $100.00 each

Available Equipment and Resources

▼ ► Bead technology (1)

For iLab Support