Welcome!
Provides standardized, high-quality services to support translational research, including pre-analytical biospecimen single cell processing and isolation procedures, as well as analytical technologies that deliver a systems-oriented perspective on the composition of a sample. Bulk sample platforms include Luminex bead arrays, high throughput pyrosequencing, and WES capillary Westerns. Services tailored for characterization of single cells include the following platforms: immunofluorescence micromanipulator manual single cell isolation, DEPArray automated single cell isolation, Biomark microfluidic PCR, and atomic force microscopy. Single-cell based informatics services are also available.
Nameer Kirma Ph.D., Director
Phone: (210) 562-4068
James L. Morris Ph.D., Laser Capture Manager
Phone: (210) 567-8389
Email: MorrisJL@uthscsa.edu
| Hours | Location |
|
Monday - Friday 8AM-5PM
|
South Texas Research Facility |
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Nameer Kirma, Ph.D. |
Director
|
(210) 562-4068
|
kirma@uthscsa.edu
|
STRF 257
|
| Chiou-Miin Wang, Ph.D. |
Lab Manager
|
(210) 562-4068
|
wangc10@uthscsa.edu
|
STRF 257
|
| Nicholas Lucio |
Staff
|
(210) 562-4068
|
luciond@uthscsa.edu
|
STRF 257
|
| Services & Rates |
| ► Fluidigm Biomark HD (6) | |||
| Name | Description | Price | |
|---|---|---|---|
| Biomark Fluidigm - Taqman Gene Expression |
Biomark Fluidigm - Taqman Gene Expression (inquire about individual probe cost)
96x96 IFC |
Internal
$1,525.00
each
External – Academic $1,982.50 each External – Commercial $3,050.00 each UT System/In Network $1,525.00 each |
|
| Biomark Fluidigm 12.765 Digital PCR |
Biomark Fluidigm 12.765 Digital PCR
12x765 IFC |
Internal
$602.00
each
External – Academic $782.60 each External – Commercial $1,204.00 each UT System/In Network $602.00 each |
|
| Biomark Fluidigm-Eva Green Gene Expression |
Biomark Fluidigm-Eva Green Gene Expression (inquire about individual probe pricing)
48x48 IFC |
Internal
$823.00
each
External – Academic $1,069.90 each External – Commercial $1,646.00 each UT System/In Network $823.00 each |
|
| Biomark Fluidigm-Eva Green Gene Expression (Bulk service > 10 chips) |
Biomark Fluidigm-Eva Green Gene Expression
48x48 IFC |
Inquire | |
| Biomark Fluidigm-Genotyping |
Biomark Fluidigm-Genotyping (inquire about SNP probe costs)
48x48 IFC |
Internal
$643.00
each
External – Academic $835.90 each External – Commercial $1,286.00 each UT System/In Network $643.00 each |
|
| Biomark Fluidigm-Taqman Gene Expression |
Biomark Fluidigm-Taqman Gene Expression (inquire about individual probe cost)
48x48 IFC |
Internal
$773.00
each
External – Academic $1,004.90 each External – Commercial $1,546.00 each UT System/In Network $773.00 each |
|
| ► Luminex FlexMap 3D (7) | |||
| Name | Description | Price | |
| Human Cytokine/Chemokine Panel 1 (without PDGF/AA, PDGF AB/BB, and RANTES) |
Human Cytokine/Chemokine Panel 1 (without PDGF/AA, PDGF AB/BB, and RANTES) |
Inquire | |
| Luminex Custom Kits |
Cost will vary for each type of custom kit |
Inquire | |
| Luminex Sample Preparation - 1 plate |
Internal
$50.00
each
External – Academic $75.00 each External – Commercial $100.00 each UT System/In Network $50.00 each |
||
| MCYTMAG-70K-PX32 | MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel - Premixed 32 Plex | Inquire | ||
| MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel | HCYTMAG-60K-41 |
Internal
$3,118.50
each
External – Academic $3,581.00 each External – Commercial $4,132.00 each UT System/In Network $3,118.50 each |
||
| Reading 1 96 well plate/Assay + Preparation by BASiC Personnel (kit NOT purchased through BASiC) |
Reading 1 96 well plate/Assay + Preparation by CATT Personnel (kit NOT purchased through BASiC)
Price per 96-Well plate |
Internal
$480.00
each
External – Academic $624.00 each External – Commercial $960.00 each UT System/In Network $480.00 each |
|
| Reading 1 96 well plate/Assay + Preparation by BASiC Personnel (must purchase kit through BASiC) |
Reading 1 96 well plate/Assay + Preparation by CATT Personnel (must purchase kit through BASIC)
Price per 96-Well plate |
Internal
$250.00
each
External – Academic $325.00 each External – Commercial $500.00 each UT System/In Network $250.00 each |
|
| ► Atomic Force Micrscopy (AFM) (7) | |||
| Name | Description | Price | |
| AFM chemical imaging with modified probes | Inquire | ||
| AFM imaging: force volume, tapping, or single point measurements | Inquire | ||
| AFM Imaging: Topography | Inquire | ||
| AFM Imaging: Topography + Mechanical Properties | Inquire | ||
| AFM Mechanical Imaging (QNM) | Inquire | ||
| AFM specialized post acquisition analysis (Morphometry, Nanomechanical Properties) | Inquire | ||
| Publication ready AFM images | Inquire | ||
| ► DEPArray (1) | |||
| Name | Description | Price | |
| Post-isolation analysis - image aquisition | Inquire | ||
| ► DNA Methylation and SNP Analysis by Pyrosequencing (8) | |||
| Name | Description | Price | |
| 96-Well Pyromark Sample Run |
96-Well plate |
Internal
$200.00
each
External – Academic $260.00 each External – Commercial $400.00 each UT System/In Network $200.00 each |
|
| Bisulfite Conversion |
Price Per Sample |
Internal
$6.50
each
External – Academic $8.30 each External – Commercial $13.00 each UT System/In Network $6.50 each |
|
| FFPE Tissue Processing (genomic DNA extraction) |
Price Per Sample |
Internal
$6.40
each
External – Academic $8.30 each External – Commercial $12.80 each UT System/In Network $6.40 each |
|
| Frozen Tissue/Cell Line Processing |
Price Per Sample |
Internal
$4.50
each
External – Academic $5.85 each External – Commercial $9.00 each UT System/In Network $4.50 each |
|
| PCR for PyroMark sample run | Inquire | ||
| Primer Assay Design and Optimization - New Primer Design and Optimization |
For each primer set |
Internal
$250.00
each
External – Academic $325.00 each External – Commercial $500.00 each UT System/In Network $250.00 each |
|
| Primer Assay Design and Optimization - Qiagen predesigned |
For each primer set |
Internal
$185.00
each
External – Academic $241.00 each External – Commercial $370.00 each UT System/In Network $185.00 each |
|
| Project Planning and Setup |
Price Per Project |
Internal
$100.00
each
External – Academic $130.00 each External – Commercial $200.00 each UT System/In Network $100.00 each |
|
| ► DNA/RNA Bioanalyzer (1) | |||
| Name | Description | Price | |
| DNA/RNA Bioanalyzer |
(cost of one chip=12 samples) |
Inquire | |
| ► Laser Capture Microscope (2) | |||
| Name | Description | Price | |
| Laser Capture Microscope (training) | Inquire | ||
| Laser Capture Microscope-trained user | Inquire | ||
| ► Miscellaneous Project Orders (1) | |||
| Name | Description | Price | |
| Reagents/Kits/Primers |
Reagents/Kits/Primers (Inquire about pricing) |
Internal
$1.00
each
External – Academic $1.30 each External – Commercial $2.00 each UT System/In Network $1.00 each |
|
| ► Nucleic Acid/Protein Quantification (2) | |||
| Name | Description | Price | |
| Nanodrop 2000 Spectrophotometer |
Price per sample |
Internal
$1.00
each
External – Academic $1.30 each External – Commercial $2.00 each UT System/In Network $1.00 each |
|
| Qubit 2.0 Fluorometer |
Price per sample |
Internal
$1.00
each
External – Academic $1.30 each External – Commercial $2.00 each UT System/In Network $1.00 each |
|
| ► Primer Design/Order (1) | |||
| Name | Description | Price | |
| Oligo Primer Design | Inquire | ||
| ► Qiagen PyroMark Q96 MD Pyrosequencing (3) | |||
| Name | Description | Price | |
| New designed assay primer testing & PCR |
Price per primer set |
Internal
$225.00
each
External – Academic $292.50 each External – Commercial $450.00 each UT System/In Network $225.00 each |
|
| Project Consultation- Pyromark |
Per Project |
Internal
$100.00
each
External – Academic $130.00 each External – Commercial $200.00 each UT System/In Network $100.00 each |
|
| Qiagen assay primer testing and PCR |
Per Primer Set |
Internal
$177.00
each
External – Academic $230.10 each External – Commercial $354.00 each UT System/In Network $177.00 each |
|
| ► Bead technology (1) | ||||||
|
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