Welcome!
Provides standardized, high-quality services to support translational research, including pre-analytical biospecimen single cell processing and isolation procedures, as well as analytical technologies that deliver a systems-oriented perspective on the composition of a sample. Analyses focus on single-cell genomics, targeted gene expression in cell populations, and single-cell proteomics. Bulk sample platforms include Luminex bead arrays, high throughput pyrosequencing, and WES capillary Westerns. Services tailored for characterization of single cells include the following platforms: immunofluorescence micromanipulator manual single cell isolation, C1 automated single-cell isolation/processing system, Biomark microfluidic PCR, the Helios CYTOF mass cytometry platform, and atomic force microscopy for nanophysical characterization of cells. Single-cell based informatics services are also available.
The single-cell core facility is funded by the Cancer Prevention Research Institute of Texas: RP150600
Nameer Kirma Ph.D., Director
Phone: (210) 562-4068
James L. Morris Ph.D., Laser Capture Manager
Phone: (210) 567-8389
Email: MorrisJL@uthscsa.edu
| Hours | Location |
|
Monday - Friday 8AM-5PM
|
South Texas Research Facility |
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Nameer Kirma, Ph.D. |
Director
|
(210) 562-4068
|
kirma@uthscsa.edu
|
STRF 257
|
| Chiou-Miin Wang, Ph.D. |
Lab Manager
|
(210) 562-4068
|
wangc10@uthscsa.edu
|
STRF 257
|
| Nicholas Lucio |
Staff
|
(210) 562-4068
|
luciond@uthscsa.edu
|
STRF 257
|
| Services & Rates |
| ► Fluidigm Biomark HD (6) | |||
| Name | Description | Price | |
|---|---|---|---|
| Biomark Fluidigm - Taqman Gene Expression |
Biomark Fluidigm - Taqman Gene Expression (inquire about individual probe cost)
96x96 IFC |
Inquire | |
| Biomark Fluidigm 12.765 Digital PCR |
Biomark Fluidigm 12.765 Digital PCR
12x765 IFC |
Inquire | |
| Biomark Fluidigm-Eva Green Gene Expression |
Biomark Fluidigm-Eva Green Gene Expression (inquire about individual probe pricing)
48x48 IFC |
Inquire | |
| Biomark Fluidigm-Eva Green Gene Expression (Bulk service > 10 chips) |
Biomark Fluidigm-Eva Green Gene Expression
48x48 IFC |
Inquire | |
| Biomark Fluidigm-Genotyping |
Biomark Fluidigm-Genotyping (inquire about SNP probe costs)
48x48 IFC |
Inquire | |
| Biomark Fluidigm-Taqman Gene Expression |
Biomark Fluidigm-Taqman Gene Expression (inquire about individual probe cost)
48x48 IFC |
Inquire | |
| ► Luminex FlexMap 3D (3) | |||
| Name | Description | Price | |
| Luminex Reading 1 96 well plate/Assay + Preparation by BASiC Personnel (must purchase kit through BASiC) |
Reading 1 96 well plate/Assay + Preparation by BASiC Personnel (must purchase kit through BASIC)
Price per 96-Well plate |
Inquire | |
| MCYTMAG-70K-PX32 | MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel - Premixed 32 Plex | Inquire | ||
| Reading 1 96 well plate/Assay + Preparation by BASiC Personnel (kit NOT purchased through BASiC) |
Reading 1 96 well plate/Assay + Preparation by BASiC Personnel (kit NOT purchased through BASiC)
Price per 96-Well plate |
Inquire | |
| ► Atomic Force Micrscopy (AFM) (7) | |||
| Name | Description | Price | |
| AFM chemical imaging with modified probes | Inquire | ||
| AFM imaging: force volume, tapping, or single point measurements | Inquire | ||
| AFM Imaging: Topography | Inquire | ||
| AFM Imaging: Topography + Mechanical Properties | Inquire | ||
| AFM Mechanical Imaging (QNM) | Inquire | ||
| AFM specialized post acquisition analysis (Morphometry, Nanomechanical Properties) | Inquire | ||
| Publication ready AFM images | Inquire | ||
| ► Automated Capillary Western Blot (1) | |||
| Name | Description | Price | |
| WES (Automated Capillary Western Blot) |
Protein Simple Technology using Capillary science Run 24 indivdiual samples in a single run |
Inquire | |
| ► C1 single-cell processing (3) | |||
| Name | Description | Price | |
| C1 HT800 single-cell mRNA Seq (includes chip and reagent costs and labor) |
C1 (Fluidigm) HT800 single-cell mRNA Seq - through pooled indexing of single-cell capture (Does not include library preparation) Note: Sequencing will be perfromed by the UTHSA Next Generation Sequencing Facility (additional costs apply) |
Inquire | |
| C1 Preamp Single-cell Processing for single-cell multiplexing PCR (includes chip, reagents and labor) |
C1 cell capture and cDNA preamplification for subsequent single-cell PCR (Up to 96 cells per chip). Does not include Fluidigm Biomark HD processing and PCR (see the Biomark services for microfluidic based single-cell PCR). |
Inquire | |
| C1 Single-Cell SMART-seq Processing 96-cells for RNA-seq (includes C1 chip, C1 single-cell processing , SMART-seq prep reagents and labor) |
-includes C1 chip, C1 single-cell processing (up to 96-cells), SMART-seq prep reagents and labor -Does not include barcoding and library construction (see prices for library construction under: C1 single-cell SMART-seq 96-cell library construction) |
Inquire | |
| ► DNA Methylation and SNP Analysis by Pyrosequencing (7) | |||
| Name | Description | Price | |
| 96-Well Pyromark Sample Run |
96-Well plate |
Inquire | |
| Bisulfite Conversion |
Price Per Sample |
Inquire | |
| FFPE Tissue Processing (genomic DNA extraction) |
Price Per Sample |
Inquire | |
| Frozen Tissue/Cell Line Processing |
Price Per Sample |
Inquire | |
| PCR for PyroMark sample run | Inquire | ||
| Primer Assay Design and Optimization - New Primer Design and Optimization |
For each primer set |
Inquire | |
| Primer Assay Design and Optimization - Qiagen predesigned |
For each primer set |
Inquire | |
| ► DNA/RNA Bioanalyzer (1) | |||
| Name | Description | Price | |
| DNA/RNA Bioanalyzer |
(cost of one chip=12 samples) |
Inquire | |
| ► Helios CyTOF (8) | |||
| Name | Description | Price | |
| Helios CyTOF Antibody Conjugation |
Pricing for Antibody conjugation of a single antibody to a single metal tag Preformed by BASiC Staff Does not include antibody Price |
Inquire | |
| Helios CyTOF Antibody Conjugation Training |
BASiC Staff will provide a one time training conjugation of a single antibody to a single metal tag Does not include cost of antibody |
Inquire | |
| Helios CyTOF Bioinformatics |
BASiC will provide basic bioInformatic analysis Inquire about details |
Inquire | |
| Helios CyTOF BioRespository Antibody Usage |
Price per antibody per sample using BASiC Core conjugation antibodies Inquire for list of antibodies and tags |
Inquire | |
| Helios CyTOF Human Immune Monitoring Panel |
BASIC Staff will perform Staining, and Running of PBMC's for a Single Sample using Maxpar Human Immune Monitoring panel (29 Antibody Marker) Inquire for the 29 Markers |
Inquire | |
| Helios CyTOF Instrument Run Time |
Run time for 4 Samples on the Helios CyTOF |
Inquire | |
| Helios CyTOF Staining |
Staining of 4 Samples with corresponding conjugated antibodies Preformed by BASiC Staff |
Inquire | |
| Helios CyTOF Staining Training |
BASiC Staff will provide a one time training of staining of a single sample with corresponding conjugated antibodies |
Inquire | |
| ► Laser Capture Microscope (2) | |||
| Name | Description | Price | |
| Laser Capture Microdissection (training) | Inquire | ||
| Laser Capture Microscope-trained user | Inquire | ||
| ► Bead technology (1) | ||||||
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