Welcome!
ALERT In Observance of U.S. Holiday, Memorial Day, Agilent Cross Lab/ iLab Operations Software Support Help Desk will be closed during U.S. hours on Monday May 25th, 2020. We will resume regular U.S. support hours on Tuesday May 26th, 2020. EU and APAC Support will remain open during this time. For urgent matters, please add "Urgent" to the ticket/ email subject or press "1", when prompted, to escalate a call on the iLab Support phone and we will prioritize those requests first.
Provides standardized, high-quality services to support translational research, including pre-analytical biospecimen single cell processing and isolation procedures, as well as analytical technologies that deliver a systems-oriented perspective on the composition of a sample. Analyses focus on single-cell genomics, targeted gene expression in cell populations, and single-cell proteomics. Bulk sample platforms include Luminex bead arrays, high throughput pyrosequencing, and WES capillary Westerns. Services tailored for characterization of single cells include the following platforms: immunofluorescence micromanipulator manual single cell isolation, C1 automated single-cell isolation/processing system, Biomark microfluidic PCR, the Helios CYTOF mass cytometry platform, and atomic force microscopy for nanophysical characterization of cells. Single-cell based informatics services are also available.
The single-cell core facility is funded by the Cancer Prevention Research Institute of Texas: RP150600
Nameer Kirma Ph.D., Director
Phone: (210) 562-4068
James L. Morris Ph.D., Laser Capture Manager
Phone: (210) 567-8389
Email: MorrisJL@uthscsa.edu
| Hours | Location |
|
Monday - Friday 8AM-5PM
|
South Texas Research Facility |
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Nameer Kirma, Ph.D. |
Director
|
(210) 562-4068
|
kirma@uthscsa.edu
|
STRF 257
|
| Chiou-Miin Wang, Ph.D. |
Lab Manager
|
(210) 562-4068
|
wangc10@uthscsa.edu
|
STRF 257
|
| Nicholas Lucio |
Staff
|
(210) 562-4068
|
luciond@uthscsa.edu
|
STRF 257
|
| Services & Rates |
| ► Fluidigm Biomark HD (6) | |||
| Name | Description | Price | |
|---|---|---|---|
| Biomark Fluidigm - Taqman Gene Expression |
Biomark Fluidigm - Taqman Gene Expression (inquire about individual probe cost)
96x96 IFC |
Inquire | |
| Biomark Fluidigm 12.765 Digital PCR |
Biomark Fluidigm 12.765 Digital PCR
12x765 IFC |
Inquire | |
| Biomark Fluidigm-Eva Green Gene Expression |
Biomark Fluidigm-Eva Green Gene Expression (inquire about individual probe pricing)
48x48 IFC |
Inquire | |
| Biomark Fluidigm-Eva Green Gene Expression (Bulk service > 10 chips) |
Biomark Fluidigm-Eva Green Gene Expression
48x48 IFC |
Inquire | |
| Biomark Fluidigm-Genotyping |
Biomark Fluidigm-Genotyping (inquire about SNP probe costs)
48x48 IFC |
Inquire | |
| Biomark Fluidigm-Taqman Gene Expression |
Biomark Fluidigm-Taqman Gene Expression (inquire about individual probe cost)
48x48 IFC |
Inquire | |
| ► Luminex FlexMap 3D (2) | |||
| Name | Description | Price | |
| FlexMap 3D Reading 1- 96 well plate with assay prepration by BASiC |
Reading 1 96 well plate/Assay + Preparation by BASiC Personnel (must purchase kit through BASIC)
Price per 96-Well plate |
Inquire | |
| Reading 1 96 well plate/Assay + Preparation by BASiC Personnel (kit NOT purchased through BASiC) |
Reading 1 96 well plate/Assay + Preparation by BASiC Personnel (kit NOT purchased through BASiC)
Price per 96-Well plate |
Inquire | |
| ► Atomic Force Micrscopy (AFM) (7) | |||
| Name | Description | Price | |
| AFM chemical imaging with modified probes | Inquire | ||
| AFM imaging: force volume, tapping, or single point measurements | Inquire | ||
| AFM Imaging: Topography | Inquire | ||
| AFM Imaging: Topography + Mechanical Properties | Inquire | ||
| AFM Mechanical Imaging (QNM) | Inquire | ||
| AFM specialized post acquisition analysis (Morphometry, Nanomechanical Properties) | Inquire | ||
| Publication ready AFM images | Inquire | ||
| ► Automated Capillary Western Blot (1) | |||
| Name | Description | Price | |
| WES (Automated Capillary Western Blot) |
Protein Simple Technology using Capillary science Run 24 indivdiual samples in a single run |
Inquire | |
| ► C1 single-cell processing (4) | |||
| Name | Description | Price | |
| C1 HT800 single-cell mRNA Seq (includes chip and reagent costs and labor) |
C1 (Fluidigm) HT800 single-cell mRNA Seq - through pooled indexing of single-cell capture (Does not include library preparation) Note: Sequencing will be perfromed by the UTHSA Next Generation Sequencing Facility (additional costs apply) |
Inquire | |
| C1 Preamp Single-cell Processing for single-cell multiplexing PCR (includes chip, reagents and labor) |
C1 cell capture and cDNA preamplification for subsequent single-cell PCR (Up to 96 cells per chip). Does not include Fluidigm Biomark HD processing and PCR (see the Biomark services for microfluidic based single-cell PCR). |
Inquire | |
| C1 Single-Cell SMART-seq Processing 96-cells for RNA-seq (includes C1 chip, C1 single-cell processing , SMART-seq prep reagents and labor) |
-includes C1 chip, C1 single-cell processing (up to 96-cells), SMART-seq prep reagents and labor
|
Inquire | |
| C1 Single-Cell SMART-seq Processing 96-cells for RNA-seq (includes C1 chip, C1 single-cell processing , SMART-seq prep reagents and labor, Barcoding Library Prep) |
Includes C1 chip, C1 single-cell processing (up to 96-cells), SMART-seq prep reagents and labor Includes Barcoding and Library prep |
Inquire | |
| ► DNA Methylation and SNP Analysis by Pyrosequencing (7) | |||
| Name | Description | Price | |
| Bisulfite Conversion |
Price Per Sample |
Inquire | |
| FFPE Tissue Processing (genomic DNA extraction) |
Price Per Sample |
Inquire | |
| Frozen Tissue/Cell Line Processing |
Price Per Sample |
Inquire | |
| PCR for PyroMark sample run | Inquire | ||
| Primer Assay Design and Optimization - New Primer Design and Optimization |
For each primer set |
Inquire | |
| Primer Assay Design and Optimization - Qiagen predesigned |
For each primer set |
Inquire | |
| Pyromark Sample Run |
Price per Sample |
Inquire | |
| ► DNA/RNA Bioanalyzer (1) | |||
| Name | Description | Price | |
| DNA/RNA Bioanalyzer |
(cost of one chip=12 samples) |
Inquire | |
| ► Helios CyTOF (8) | |||
| Name | Description | Price | |
| Helios CyTOF Antibody Conjugation |
Pricing for Antibody conjugation of a single antibody to a single metal tag Preformed by BASiC Staff Does not include antibody Price |
Inquire | |
| Helios CyTOF Antibody Conjugation Training |
BASiC Staff will provide a one time training conjugation of a single antibody to a single metal tag Does not include cost of antibody |
Inquire | |
| Helios CyTOF Bioinformatics |
BASiC will provide basic bioInformatic analysis Inquire about details |
Inquire | |
| Helios CyTOF BioRespository Antibody Usage |
Price per antibody per sample using BASiC Core conjugation antibodies Inquire for list of antibodies and tags |
Inquire | |
| Helios CyTOF Human Immune Monitoring Panel |
BASIC Staff will perform Staining, and Running of PBMC's for a Single Sample using Maxpar Human Immune Monitoring panel (29 Antibody Marker) Inquire for the 29 Markers |
Inquire | |
| Helios CyTOF Instrument Run Time |
Run time for 4 Samples on the Helios CyTOF |
Inquire | |
| Helios CyTOF Staining |
Staining of 4 Samples with corresponding conjugated antibodies Preformed by BASiC Staff |
Inquire | |
| Helios CyTOF Staining Training |
BASiC Staff will provide a one time training of staining of a single sample with corresponding conjugated antibodies |
Inquire | |
| ► Laser Capture Microscope (2) | |||
| Name | Description | Price | |
| Laser Capture Microdissection (training) | Inquire | ||
| Laser Capture Microscope-trained user | Inquire | ||
| ► Bead technology (1) | ||||||
|
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